Question: How to use WEGO (Web Gene Ontology Annotation Plot)
gravatar for amyfm
5.0 years ago by
amyfm10 wrote:


I have an excell file with around 1000 genes of interest to me and I want to do pathway analysis. I read about WEGO (, an online tool to get nice graphics of the pathways, but I don't know how to use it. I don't know which file I should upload.

Previously, I go to gene ontology website, and I use Panther classification system to get information about cellular process, molecular functions, etc. I download some output files and upload them to WEGO but it is not working.

Can anybody explain to me how to use WEGO starting from an excel file with genes?


Thank you

wego gene ontology pathway • 4.5k views
ADD COMMENTlink modified 2.8 years ago by lakhujanivijay5.3k • written 5.0 years ago by amyfm10
gravatar for Guangchuang Yu
5.0 years ago by
Guangchuang Yu2.4k
China/Guangzhou/Southern Medical University
Guangchuang Yu2.4k wrote:

wego stop updating their data since 2009. see

I recommend you not to use it.

ADD COMMENTlink modified 11 months ago by RamRS30k • written 5.0 years ago by Guangchuang Yu2.4k
gravatar for lakhujanivijay
2.8 years ago by
lakhujanivijay5.3k wrote:

This is an update that I wanted to share. It seems that the wego wesite got a makeover recently and appears quite better

Just have a look

Though I am still struggling to get a good quality bar plot in png format.

ADD COMMENTlink written 2.8 years ago by lakhujanivijay5.3k
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