Pathogen Identification via Oligo-Array
Entering edit mode
6.9 years ago

Dear All

For my phd THESÄ°S;

I designed microarray assay for detecting pathogens(15 pathogen including gram + and gram -) from blood cultures and need suggestions;

I have two steps on the experiment.

  1. Amplification of 300bp amplicon of gyrB gene (by using only 1 primer pair/ including degenerated mix bases of course) of pathogens DNA isolated from positive cultures
  2. Using fluoresecence microarray analyzer for detecting the hybridized probes.

I got stuck on the second step. I can't decide. to the oligonucleotide probes that will be attached to the slide :(

I mean, I can select the probes manually ( 20-25bp) and confirm them by my own EYEs ON THE ALIGNMENT FILES. But I would like to check via confirmational softwares for more details on analytical points.

Any experienced helpers?


Looking forward


oligonucleotide-probes microarray alignment • 1.1k views

Login before adding your answer.

Traffic: 1056 users visited in the last hour
Help About
Access RSS

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6