Question: Pathogen Identification via Oligo-Array
gravatar for mr.donmez.emre
5.0 years ago by
mr.donmez.emre0 wrote:

Dear All

For my phd THESİS;

I  designed microarray assay for detecting pathogens(15 pathogen including gram + and gram -) from blood cultures and need suggestions;

I have two steps on the experiment.

     1-amplification of 300bp amplicon of  gyrB gene ( by using only 1 primer pair/ including degenerated mix bases of course) of pathogens DNA isolated from positive cultures

     2- using fluoresecence microarray analyzer for detecting the hybridized probes.

i got stucked on the second step. I cant decide. to the oligonucleotide probes that willl be attached to the slide :(

i mean , i can select the probes manually ( 20-25bp) and confirm them by my own EYEs ON THE ALIGNMENT FILES. But I would like to check via confirmational softwares for more details on analytical points.

any experinced helpers??????




looking forward



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