Off topic:variant calling based on ab1 file from Sanger sequencing

Question

[J.F.Jiang]

Hi all,

We have use Sanger to verify the variants that are discovered by NGS platform.

Since there are lots of such issues, it is hard for us to look for these variants manually one by one.

Is there any tool that can automatically generate the variant calling results based on ab1 file only?

PS, I've tried novoSNP, but it does not work after loading ref and runs.

Thanks.

Junfeng