There is a lot of interest in tissue-specific and differentially expressed genes and a lot of people are using RNAseq to determine if genes are upregulated in one tissue vs. another. However, I am interested in a particular gene that, based on qPCR, is highly expressed in a particular aphid tissue (bacteriocyte), but it is not differentially expressed (it is also highly expressed overall in the body and in other tissues, like head and gut). So I'm not interested in using RNAseq to measure differential expression of this gene, because I don't necessarily expect to find it. I am interested in using RNAseq to confirm high expression in bacteriocyte (relative to other genes in the same gene family). Is there a way to do this? I know that RNAseq does not measure absolute expression, but would it be vaild to rank some measure of expression (like TPM) of, say, my gene of interest and other members of the same gene family within bacteriocyte? I would like to know if this gene and its orthologs are, or are not, highly expressed in bacteriocyte tissues relative to other genes in the same gene family. In theory, I would like to be able to quantitatively compare across orthologs/different insects, but I would be happy with independent yes/no answers for each insect.