Hi every one,
I am new in bioinformatic and NGS field .So i have some points which i confused in and hope to find some answers ..
First: when we study gene expression why some times we do assembly after sequencing then qrt-pcr and some times only sequencing and qrt-pcr ??cant understand this point
Second:Why when we study gene expression some times we do biological replication when we do sequencing and some times without biological replications?!!