Question: Running BLAST+(version 2.2.31) locally for GRCH37 refseq
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gravatar for niharraul
3.9 years ago by
niharraul0
United Kingdom
niharraul0 wrote:

I am trying to use BLAST updateblastdb.pl refseq_genomic script to download human genomic datasets so that I can run the BLAST software locally. However, I realized these datasets would be for GRCH38 (the latest versions) and not GRCH37.

  1. My question is to how to get the files for GRCH37?
  2.  What command should I be using to run it locally. Since I will be aligning a chunk of different DNA sequences(querry sequences could be upto 5000 in number) of various length  from 20 bp to say 120 bp? Since if using protein database  blastp is used, blast for nucleotide database  and etc. 

 

blast alignment genome • 1.3k views
ADD COMMENTlink modified 3.9 years ago • written 3.9 years ago by niharraul0
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