i have a few rookie questions.
i have a several hundreds of transcripts which are up regulated in an insect response to low temperature.
i wanna use DAVID(https://david.ncifcrf.gov/home.jsp) analyse them.
But when i looked at DAVID, it receives genes in list of their ENTREZ_ID and OFFICIAL_GENE_SYMBOL (i assume gene symbol refers to gene name right?).
i have annotated those sequences by using blast2go before, but the software gives the sequence's description, not gene name or seq ID.
so what i wanna ask here is how can i get my sequence corresponding ENTREZ ID, gene symbol (name) or any information like that from database like NCBI.
I thought may be i could get those information from blasting my sequences with NCBI nr.fasta data, but when i see that the whole file in NCBI the compressed version of the file is around 14G, then i think i can not do that on laptop, do i?
could you please give me some tips on those, which could really help me with the downstream analysis of those sequences.