read length versus unique alignment rate
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5.9 years ago
igor 12k

How does the read sequence length affect alignment rates? It's almost impossible to have 10 bp align uniquely, but 100 bp works fairly well. I was hoping to find some table or chart that may answer this question more definitively, but haven't been able to do so on my own.

I am mostly concerned in the context of read trimming and setting the minimum read length for mammalian genomes, but any info would be helpful.

sequencing alignment • 2.1k views
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