Question: What library fits best for MNase ChIP experiments?
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gravatar for mkrause
4.0 years ago by
mkrause30
Germany
mkrause30 wrote:

Hello Community.
I am facing problems with mappability after doing MNase ChIP-Seq.
Somehow I enrich for multimapper fraction in Seq results.
The quality and complexity of the reads are fine, but multi mappers are overrepresented (70-80%).

I wonder whether this could be due to not-fitting library prep.

Does anyone has experience on which library prep kit fits best for MNase experiments?

Thanks

chip-seq mnase multi-mapping • 881 views
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