Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : all time
all time
today
this week
this month
this year
116,594 results • Page
1 of 2332
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
1
view
Highest variable features in single cell data
cell
single
just now by
Kazo
• 0
0
votes
0
replies
11
views
Hide positions in alignment with 99% "–" characters to ignore single sequence insertions?
alignment
gaps
31 minutes ago by
Broccoli
• 0
0
votes
0
replies
19
views
Fetch table from clinvar database according to a list of rsid
clinvar
fetching
data
python
perl
31 minutes ago by
ashaneev07
▴ 20
0
votes
1
reply
92
views
Network Alignment
plugin
Cytoscape
CytoMCS
updated 2 hours ago by
Scooter
▴ 280 • written 22 hours ago by
Akash D
▴ 40
2
votes
4
replies
2.3k
views
How could I generate a gi_taxid_nucl.dmp file similar to the one previously hosted by NCBI?
ncbi
metagenomics
centrifuge
gi_taxid_nucl.dmp
taxonomy
updated 3 hours ago by
ZhangYuanfeng
• 0 • written 2.4 years ago by
Frazier Baker
• 0
0
votes
0
replies
34
views
PCA plot
DESeq2
PCAplot
3 hours ago by
Aaliya
▴ 10
0
votes
0
replies
26
views
Tutorial:
how to combine multiple RNAseq count files into a single dataframe in R and unix
Unix
RNAseq
R
3 hours ago by
Ming Tommy Tang
★ 3.9k
0
votes
3
replies
228
views
Heatmap and rna-seq
RNA-Seq
Heatmap
and
updated 4 hours ago by
Kevin Blighe
87k • written 2 days ago by
qudrat.nii
▴ 10
795
votes
167
replies
141k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.7k • written 7.4 years ago by
Istvan Albert
100k
0
votes
4
replies
2.6k
views
Lositan freezing when generating selection table
LOSITAN
updated 5 hours ago by
evawillms183
• 0 • written 8.3 years ago by
andrepleao
• 0
0
votes
0
replies
50
views
Can you give me an example of a small globular protein (PDB) that has disordered alpha-carbons?
protein
pdb
alpha-carbon
6 hours ago by
4fzcgueyp5
• 0
2
votes
3
replies
208
views
clustalo - align milions of short seq, memory issue
memory
clustalo
updated 2 days ago by
Mensur Dlakic
★ 27k • written 2 days ago by
emmanouil.a
▴ 120
0
votes
1
reply
100
views
Annovar using R package
Annovar
gnomAD
R
updated 8 hours ago by
Pierre Lindenbaum
161k • written 11 hours ago by
DKA
▴ 40
0
votes
2
replies
130
views
Creating Synthetic Sequences for a ML Model
DNA
ML
updated 9 hours ago by
Mensur Dlakic
★ 27k • written 12 hours ago by
biochugs
• 0
3
votes
8
replies
368
views
PCA plot (Suggestions Needed)
DESeq2
PCAplot
updated 1 hour ago by
swbarnes2
14k • written 1 day ago by
Aaliya
▴ 10
0
votes
6
replies
632
views
Adding CB tag to bam file
samtools
bam
updated 10 hours ago by
swbarnes2
14k • written 6 days ago by
Maria
• 0
1
vote
10
replies
849
views
Troubleshooting RNA-seq data with DNA contamination
RNA-seq
DESeq2
DNA-contamination
11 hours ago by
DKA
▴ 40
0
votes
0
replies
61
views
Finding orthologs between genome error
OMA
OMAbrowser
13 hours ago by
hemantcnaik
• 0
0
votes
2
replies
742
views
AGeNT LocatIt
AGeNT
LocatIt
WES
UMI
duplicates
updated 14 hours ago by
barslmn
★ 2.1k • written 2.8 years ago by
jhy
▴ 10
0
votes
3
replies
154
views
Is it possible to get a list of representative genomes from a past RefSeq release?
representative
ncbi
asembly
refseq
updated 15 hours ago by
GenoMax
141k • written 16 hours ago by
Bertalan_Takacs
▴ 90
0
votes
0
replies
73
views
One error problem occurred caused by data type during using GDCprepare to handle BRCA data
TCGA
GDCprepare
updated 11 hours ago by
Ram
43k • written 16 hours ago by
glaciya2018
• 0
0
votes
0
replies
64
views
News:
8th Berlin Summer School in NGS Data Analysis - Apply Now
variantcalling
DNAseq
illumina
RNAseq
transcriptomics
16 hours ago by
David Langenberger
11k
0
votes
0
replies
120
views
roary not working with pgap output gff files with fasta sequence but works with prokka's gff outputs
pangenome
PGAP
NCBI
roary
Prokka
17 hours ago by
pramach1
▴ 40
0
votes
14
replies
3.4k
views
6 follow
Tax4Fun2 - error
tax4fun
silva
blast
updated 18 hours ago by
drstalinantony28
• 0 • written 2.2 years ago by
ymj
▴ 10
0
votes
1
reply
101
views
GEMMA GWAS how to specify factor or numerical for covariates
covariate
GEMMA
GWAS
updated 15 hours ago by
Sofia
• 0 • written 19 hours ago by
mawigoj318
• 0
0
votes
0
replies
85
views
Sequence BLAST in plus/minus and plus/plus
blast
CRISPR
strand
sequencing
21 hours ago by
salias
• 0
4
votes
6
replies
287
views
To get p-values for the TPM
P-value
TPM
RSEM
33 minutes ago by
VITALA
• 0
0
votes
2
replies
148
views
different FeatureCounts output for the same data
fpkm
Counts
Rsubread
rna-seq
updated 12 hours ago by
Ram
43k • written 21 hours ago by
sehriban.buyukkilic
▴ 10
0
votes
0
replies
77
views
Alternative splicing analysis using SUPPA tool - how to normalise counts?
rna-seq
single-cell
suppa
alternative-splicing
updated 12 hours ago by
Ram
43k • written 22 hours ago by
newuser2024
• 0
1
vote
2
replies
124
views
alignment result
RNA-seq
samtools
hisat2
1 hour ago by
ahmad.sajad4541
• 0
0
votes
0
replies
72
views
News:
hands-on introduction to generalized linear models (GLMs) using R
Generalized-Linear-Models
GLM
R
updated 12 hours ago by
Ram
43k • written 1 day ago by
carlopecoraro2
★ 2.5k
0
votes
0
replies
68
views
Is result interpretation of the modelHomotypic function of the DoubletFinder correct?
doublet
DoubletFinder
Seurat
modelHomotypic
updated 12 hours ago by
Ram
43k • written 1 day ago by
Jeyong
• 0
0
votes
2
replies
180
views
Wilcox test using data slot (log normalized count values) of scRNA-seq between two groups
scRNAseq
18 hours ago by
mropri
▴ 150
2
votes
2
replies
164
views
HaplotypeCaller - only SNPs
HaplotypeCaller
GATK
updated 1 day ago by
analyst
▴ 30 • written 1 day ago by
lorena9132
• 0
0
votes
0
replies
79
views
AttributeError: module 'scvelo' has no attribute 'pp
loomfile
scvelo
scipy
annData
numpy
1 day ago by
Kash
▴ 110
7
votes
2
replies
229
views
Gene set enrichment analysis differences between 2020 and 2024
Gene-ontology
updated 1 day ago by
geneontologyhelp
▴ 390 • written 2 days ago by
catherine.teyssier
• 0
0
votes
1
reply
170
views
Blasting two protein sequences vs two nucleotide sequences
gene-hunting
blast
updated 1 day ago by
Istvan Albert
100k • written 2 days ago by
Abeer
• 0
0
votes
4
replies
251
views
Should I use unpaired reads from trimmomatic
RNA-seq
QC
Trimmomatic
updated 1 day ago by
swbarnes2
14k • written 2 days ago by
dxj294
• 0
0
votes
1
reply
119
views
How to solve this RoseTTAFold colaboratory error?
modeling
Google
Colaboratory
RoseTTAFold
protein
updated 1 day ago by
Mensur Dlakic
★ 27k • written 1 day ago by
benguyarenbeyaz98
• 0
0
votes
2
replies
202
views
How to calculate identity percentage between proteins contained in a FASTA file?
protein
FASTA
alignment
updated 12 hours ago by
Ram
43k • written 1 day ago by
v.berriosfarias
▴ 140
2
votes
1
reply
177
views
Seurat merge and batch correction
Seurat
updated 1 day ago by
Ram
43k • written 2 days ago by
sooni
▴ 20
1
vote
1
reply
148
views
Herald:
The Biostar Herald for Tuesday, April 23, 2024
herald
updated 1 day ago by
Pierre Lindenbaum
161k • written 1 day ago by
Biostar
2.7k
1
vote
1
reply
123
views
Including plasmid in transcriptome assemblies
Bacteria
BOWTIE2
Transcriptomics
STAR
updated 1 day ago by
GenoMax
141k • written 1 day ago by
heelpPlease
• 0
2
votes
9
replies
796
views
TCGA2STAT Error: Firehose connection
FIREHOSE
TCGA
updated 1 day ago by
LauferVA
4.2k • written 6 months ago by
Gnana
• 0
0
votes
10
replies
361
views
How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq libraries?
STARSolo
scRNA-seq
STAR
snRNA-seq
MGI
1 day ago by
atowns21
• 0
0
votes
1
reply
132
views
Find subcluster under a cluster, find differential genes in one cluster between 2 samples??
clustering
subclustering
scRNAseq
updated 1 day ago by
bk11
★ 2.3k • written 1 day ago by
alphaflylizard
• 0
3
votes
3
replies
194
views
Forum:
Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 1 day ago by
GenoMax
141k • written 1 day ago by
Estevão
• 0
0
votes
3
replies
213
views
Difference between samtools "-f 9" and "-f 11"
samtools
sam
bam
updated 1 day ago by
Istvan Albert
100k • written 1 day ago by
Dylan
• 0
1
vote
4
replies
218
views
How to trim transcripts using information from NCBI contamination screen report
RNAseq
assembly
transcriptome
contamination
updated 13 hours ago by
GenoMax
141k • written 1 day ago by
Lada
▴ 30
4
votes
11
replies
477
views
Redirection of Duplicate PMIDs
pubmed
pmid
updated 1 day ago by
LauferVA
4.2k • written 2 days ago by
dominickd
• 0
116,594 results • Page
1 of 2332
Recent Votes
Comment: alignment result
Comment: clustalo - align milions of short seq, memory issue
Answer: clustalo - align milions of short seq, memory issue
Comment: downloading genomes in fasta format from accession ids
Comment: PCA plot (Suggestions Needed)
Answer: PCA plot (Suggestions Needed)
PCA plot (Suggestions Needed)
Recent Locations •
All
Turkey,
2 minutes ago
WCIP | Glasgow | UK,
8 minutes ago
India,
11 minutes ago
Bologna,
11 minutes ago
Pakistan,
14 minutes ago
India,
14 minutes ago
Yalta, NBG-NSC,
15 minutes ago
Recent Awards •
All
Popular Question
to
anasjamshed
▴ 120
Popular Question
to
camillab.
▴ 160
Popular Question
to
Simone
▴ 10
Popular Question
to
carlopecoraro2
★ 2.5k
Popular Question
to
daffodil
▴ 10
Popular Question
to
Russ
▴ 500
Popular Question
to
pramach1
▴ 40
Recent Replies
Comment: To get p-values for the TPM
by
VITALA
• 0
yes i want what rsem does and i did try with prior-enhanced option/pRSEM, but i think pRSEM is for chipseq data
Comment: alignment result
by
ahmad.sajad4541
• 0
Thank you so much. Should i proceed for annotation step?
Comment: PCA plot (Suggestions Needed)
by
swbarnes2
14k
The code you already posted in the bioconductor board had simulated data, and the PCA image is the same.
Answer: Network Alignment
by
Scooter
▴ 280
Unfortunately, it's not working for me either, and the web site doesn't exist anymore. You might try reaching out to the authors, but I kn…
Answer: How could I generate a gi_taxid_nucl.dmp file similar to the one previously host
by
ZhangYuanfeng
• 0
JUST GIVE THE ANSWER! Here is the solution: ```bash aria2c -c -s 4 -j 4 \ https://ftp.ncbi.nlm.nih.gov/pub/taxonomy/accession2taxid/pd…
Comment: PCA plot (Suggestions Needed)
by
Aaliya
▴ 10
I have actually used DESeq2 data.
Comment: Why cd-hit-est not work when sequence identity threshold<0.95?
by
Dateline
• 0
So, why? I have 18GB of data, and when -c=0.95, the run ends in about two days, while when -c=0.9, it has been three days and only a little…
Answer: Heatmap and rna-seq
by
Kevin Blighe
87k
Hi, you can take the list of GSEA pathways and then use these via GSVA (https://github.com/rcastelo/GSVA) to create a heatmap. You hav…
Comment: Clustering of DAVID gene enrichment results from gene expression studies
by
Kevin Blighe
87k
You will have to elaborate on what is the error
Comment: clustalo - align milions of short seq, memory issue
by
emmanouil.a
▴ 120
Hi GenoMax and Mensur Dlakic, thank you for your feedback. I'm changing approach in the way to work with a smaller number of seq.
Comment: Annovar using R package
by
Pierre Lindenbaum
161k
duplicate of your previous question: https://www.biostars.org/p/9592646/ why using R when there are shell-based tools like VEP ? slivar , …
Comment: Trouble with Pilon installation
by
Lissa Cruz Saavedra
• 0
Hi I am trying to install the software but I didn't find the way to clone from github. Could you help me with the pipeline of instruction…
Comment: Adding CB tag to bam file
by
swbarnes2
14k
I think both these programs are just adding the same tag to every line. I don't think either cares whether it's called RG or CB
Comment: PCA plot (Suggestions Needed)
by
swbarnes2
14k
Even if this was real data, the tiny numbers for the PC1 and PC2 indicate that the differences between the samples are all absolutely tiny.…
Comment: Creating Synthetic Sequences for a ML Model
by
biochugs
• 0
Thanks for the input. I'll continue to think of more ideas that may be achievable.
Traffic: 2153 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6