I have cancer samples that lack germline control and I am interested to identify somatic variants as accurately as possible. I did filtering based on panel of normals and filtered out reported variants in public databases. However, I still have some suspicious cases that I want to resolve and I wonder if phasing can be used here.
Here is an IGV snapshot of an example where three nonsynonymous mutations are detected:
Can we know confidently based on phasing which of these three are somatic? If yes, is there a systematic way to do such analysis?