I read many materials regarding KOG annotation for transcriptome assembly, but I'm still confused that how to perform it! The best thing that I found is related to making KOG database (makeprofiledb -title KOG.v.1.0 -in Kog.pn -out Kog -threshold 9.82 -scale 100.0 -dbtype rps -index true). Could you please let me know if running the blastp (from ncbi-blast+ package) of a transcriptme assembly against the above-made database is a right task? if no, please advise me to correct way. I also read about psi blast, but did not find any complete tutorial or documentation about it. Having any suggestion and hand experience from you about KOG annotation would be really welcomed.