Question: ncbi-blast-2.3.0+ blastn problem
0
gravatar for akyladisla10
4.1 years ago by
akyladisla100 wrote:

Hello, 

I recently tried installing BLAST+ command line on windows and tried running a blastn command in command prompt

"blastn -query test_query.txt -db refseq_rna.00 -out test_output.txt"

giving the following error:

"Error: NCBI C++ Exception:

TO"..\..\..\..\..\src\corelib\ncbiobj.cpp", line 977: Critical: ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL pointer."

Can anyone please help me on this? Thanks.

blastn blast+ windows ncbi • 2.1k views
ADD COMMENTlink modified 4.0 years ago by Jenez520 • written 4.1 years ago by akyladisla100

Dear akyladisla10

You may go through this post

Hope it helps

ADD REPLYlink modified 4 weeks ago by RamRS25k • written 4.1 years ago by iamtuttu540

Hi! I still cant resolve this problem :( I am using 64-bit windows 8 machine. I installed ncbi-blast-2.3.0+-win64.exe and even re-installed it twice. I followed everything in this tutorial http://www.ncbi.nlm.nih.gov/books/NBK52637/

I have no idea where is the problem. Can anyone please help me ? :( thanks in advance.

ADD REPLYlink modified 4 weeks ago by RamRS25k • written 4.0 years ago by akyladisla100

It could be a bug in the latest version of blastn. Have you tried contacting their helpdesk ?
In the meantime, make sure that your test_query.txt is properly FASTA-formatted.

ADD REPLYlink written 4.0 years ago by Jean-Karim Heriche21k
1
gravatar for Jenez
4.0 years ago by
Jenez520
Sweden
Jenez520 wrote:

Not sure if relevant but try removing the zeros from refseq_rna.00

blastn -query test_query.txt -db refseq_rna -out test_output.txt

You do not need to specify the index for the database

ADD COMMENTlink modified 4 weeks ago by RamRS25k • written 4.0 years ago by Jenez520
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