We have data of tumor (Tissue) and Normal (Blood) control from the same patients. We want to call somatic and germline variants in the same individual.
To achieve this, We aligned Tumor and normal sample each separately with hg19 and called variants. For identification of somatic mutations, we compare mutations of both sample together and, mutations occurring only in tumor sample was considered to be somatic. Now for germline detection, we considered all mutations identified in Normal sample.
Is this the Wright approach for Somatic and germline identification from the same individual? or any suggestions?