I have a 86Mbp plant genome. It was assembled with very high coverage and some mate pair info for scaffolding.
After the process was finished, I ended up with 50Mbp accumulated contig size.
Someone has an idea why it is so much less? In general contigs should accumulate to a size high then the genome. Could it be caused by diploidy?
Or am I completly wrong?
EDIT:
Reads: PE Illumina 100bp, MP Illumina, 2kbp, 10kbp, 20kbp
Assembler: CLC
Scaffoler: SSpace
Coverage: 50x
Can you give us more information on what sequencing platform? How many reads? What the coverage is? What assembler you used?