I have tried several options on the web including a few post her on Biostar to split my VCF file by chromosome, but could not do it properly. Say I have a vcfile called myvcf.vcf.gz and want to split that per chromosome, what would be the best way to split it by chromosome?
bgzip -c myvcf.vcf > myvcf.vcf.gz tabix -p vcf myvcf.vcf.gz tabix myvcf.vcf.gz chr1 > chr1.vcf
It will give chr1.vcf file containing variants for chr1. You can loop the last command over all the chromosomes. If you need vcf header also, use -h flag with last command.
You can use the snpSift splitchr command. It separates a vcf file according to the chromosomes.
The command is simple:
Java -jar snpSift splitChr file.vcf
The question can be answered in a single command, using a bash loop to feed chromosome names into GATK's "select variants" command as shown below, whereby "-L" specifies which chromosome to select (https://software.broadinstitute.org/gatk/). This has the advantage over other methods in that index files are generated "on-the-fly". Also GATK is usually pretty robust. If using a genome with many chromosomes named e.g 1-22, users should modify the loop parameters, to something like "for i in seq 1 22;do"
for i in chr2L chr2R chr3L chr3R chr4 chrX;do
GenomeAnalysisTK -R ${ref_seq} \
-T SelectVariants \
-V my_flies.vcf \
-L $i \
-o my_flies.${i}.vcf
done;
That's a bash loop executing a GATK java program.
Thanks much ! Btw, I just stumbled upon https://gigabaseorgigabyte.wordpress.com/2017/05/02/an-orphan-bioinformatician/ while following your profile in biostars and then wordpress.
I am mainly a evolutionary biologist with a huge transition into Bioinformatics. If you would like to discuss about your problem I hope I can help, though I am not a full fledged programmer at this point. I have though managed to prepare a pipeline (or programme, whatever you may call it, lol) using python which is going to need a lots of cleaning and making it efficient, so I am learning. https://github.com/everestial/pHASE-Stitcher
Interesting fact is that I also consider myself a orphan bioinformatician (I like this word !). My situation was even terrible; I started PhD using genome and RNASeq data analyses, then came upon this problem of haplotype phasing in F1 hybrids, which wasn't solvable using any tools available. Additionally, there was null support on the matters of programming in my department and lab, but learning in a hardway has opened my pathway to writing my own program.
Your Biostars rep is quite high so I am thinking you might be quite ahead of me, but I think it would not hurt to discuss.
Thanks, - Bishwa K.
Thanks for reading. Feel free to comment on my blog post if you have suggestions, remarks or something else to add.
I have though managed to prepare a pipeline (or programme, whatever you may call it, lol) using python which is going to need a lots of cleaning and making it efficient, so I am learning. https://github.com/everestial/pHASE-Stitcher
That's the thing, you always keep learning and improving. Good luck writing your script!
Every now and then I have a look at old code and give it a makeover, it's surprising how much you learn in a few months.
I think the best way to help and discuss is to put your tool on github (not matter how much small it might be). I just helps me or other people to create a branch and suggest the changes. Depending on how it works out for you or how it achieves the goal of the pipeline, you can merge it into the master branch.
Are you on github? Else, I have found these to be quite helpful :)
http://product.hubspot.com/blog/git-and-github-tutorial-for-beginners
We are going fairly off topic here...
Thanks for the links, but I already have a github account and a few repositories.
You can use vcftools. For example, if total 16 chromosomes
for i in {1..16};
do vcftools --vcf VCF_FILE --chr $i --recode --recode-INFO-all --out VCF_$i;
done