I have deep RNA sequences of human one cell line (100bp,paired-end). I used the reference annotation from Gencode (version 22) for transcript assembly of my sequences and identified more than 200 annotated miRNA in my datasets. I would like to know are this miRNA are reliable?
As I did not performed small RNA sequencing , how I can report theses mi RNA?
you can't in fact if you didn't do smallRNA-Seq.