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8.3 years ago
songmm19900210
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20
Hi, I met a problem, I hava two samples,the two samples are the same species, they have a similarity of 90%,the two samples are assembled by trinity respectively, I got more than 60000, 50000 unigenes respectively. I used cd-hit to remove redundancy and tgicl to cluster unigenes. I got 90000 unigenes, most unigenes are not clustered. I blast two samples, the mapped result is 75%. Why most unigenes are not clustered?
