Off topic:Scaffold making via scripting

Question

[vidushipujni]

Hi

My question today is for experts using python programming to do Bioinformatic analyses. I am trying to run a well written script for creating scaffold from my blast sequences. I am however getting the error again and again. I have pasted the error I am getting

Traceback (most recent call last):
  File "C:/Python27/DB/Attempt3.py", line 150, in <module>
    __main__()
  File "C:/Python27/DB/Attempt3.py", line 143, in __main__
    ContigsDict = myContigs (args.sequences)
  File "C:/Python27/DB/Attempt3.py", line 49, in myContigs  with open(file, "r") as f:

TypeError: coercing to Unicode: need string or buffer, NoneType found

Can somebody please advise me why I am getting his error.I know I have installed python and biopython as I am able to parse known sequences using this.

Regards
DV