Entering edit mode
8.2 years ago
rbioinfo
▴
40
Hello,
I need to design about 50 primer pairs for multiplex PCR. The thing is I need to have approximately the same amount of product from each pair. To optimise the single pair sometimes is not a trivial but did somebody use tools for multiplex PCR like PCRTiler or anything else? Are they efficient? Or it is more reliably to construct each pair manually via CLC?
Thank you in advance