Question: Multiplex PCR primer design efficiency
gravatar for makarenko100
4.5 years ago by
makarenko10010 wrote:


I need to design about 50 primer pairs for multiplex PCR. The thing is I need to have approximately the same amount of product from each pair. To optimise the single pair sometimes is not a trivial but did somebody use tools for multiplex PCR like PCRTiler or anything else? Are they efficient? Or it is more reliably to construct each pair manually via CLC?

Thank you in advance

ADD COMMENTlink modified 4.5 years ago • written 4.5 years ago by makarenko10010
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