Hi i download a data set from SRA database get from ABI Solid 4 System platform, i used the following command: bowtie ../../Syne -C -S -p 7 --best --strata -m 1 ../stdc_1.fastq >stc_1.sam to get uniquely mapped, but after a minuts running the alingment abort showing:
Reads file contained a pattern with more than 1024 quality values.
Please truncate reads and quality values and and re-run Bowtie
terminate called after throwing an instance of 'int'
reading in forum they say that its a problem with multiples "." in a group of read, to solve that i removed that group of read and the problem persist.
Any idea of why is happening or if im doing somthing wrong?...