Question: Rna Seq Procedure For Generating Cdna Library
0
gravatar for Varun Gupta
7.2 years ago by
Varun Gupta1.1k
United States
Varun Gupta1.1k wrote:

Hi Everyone

I would like to have some descripition on making cdna libraries for RNA seq analysis. I do not want protocol for making these libraries, but figures so as to understand how ds cdna is produced , how it is end repaired, ligated to adapters etc.

Then i would like to understand how cluster formation is done(through figures)

And finally sequencing procedure (through figures).

Any figures, papers etc which explain me the details of how cdna libraries are created for rna seq would be nice.(I wiould like to have pictorial representations or videos if possible).

I do not want the protocol for this

Thanks for all the help

Regards

cdna rna • 2.4k views
ADD COMMENTlink modified 7.1 years ago by Mnkyboy60 • written 7.2 years ago by Varun Gupta1.1k
1
gravatar for Damian Kao
7.2 years ago by
Damian Kao15k
USA
Damian Kao15k wrote:

There are a lot of resources if you just google it. Here are a few from seqanswers.

Illumina: http://seqanswers.com/forums/showthread.php?t=21

ABI SOLiD: http://seqanswers.com/forums/showthread.php?t=10

ADD COMMENTlink written 7.2 years ago by Damian Kao15k
0
gravatar for Mnkyboy
7.2 years ago by
Mnkyboy60
PNW
Mnkyboy60 wrote:

Do you have a specific type of RNA-seq library you would like to know about? There are multiple preparations for mRNA-seq, Total RNA-seq, and small RNA-seq. There are also specifics about whether you want strand specific libraries.

The seqanswers links will answer a lot of these but there is more than just RNA-seq.

ADD COMMENTlink written 7.2 years ago by Mnkyboy60

mRNA-seq i am looking for

Thanks

ADD REPLYlink written 7.2 years ago by Varun Gupta1.1k
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