Question: MeDIP-seq and hMeDIP-seq analysis
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Grinch • 30 wrote:
Hi! I'm analysing MeDIP-seq and hMeDIP-seq data for the first time (I've analysed ChIP-seq and RNA-seq data sets before) and was wondering if people who have done it before can recommend pipelines they have used and/or programmes for quantitative comparison of ChIP-Seq data. My data set is made of replicates and has 2 disease subtypes and healthy samples.
I've analysed with the new MethylAction pipeline and MACS peak caller, but to compare between the two I need to choose a programme for quantitative comparison for the peaks I get with MACS, so I can see which one gives me better results for my data.
If anyone has knowledge of a better pipeline, that would also be of use, but firstly I need to quantitate the peaks.
Thank you all!
