The most popular GO annotation tool

Question

[seta]

Hi all,

I'm busy with annotation of a de novo assembled transcriptome. For GO annotation, at the first I used blast2go software with loading the blastx results of transcriptome assembly, but the mapping step was so slow. I searched on net for alternative tools and found several for this purpose, but none of them are not as well-known as blast2go. Based on your experiments, could you please suggest me the appropriate tool?

Thanks

[Jean-Karim Heriche]

It's not a popular tool nor the fastest for this purpose but the way I'd do it would be, if dealing with an animal, to assign each sequence to a TreeFam family using treefamscan and transfer GO annotations by orthology.

Answer 1

[Juke34]

You should find what you want here: A: Softwares for gene annotation