Question: (Closed) Differential Expression From The Single End Read
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Senthilkumar • 90 wrote:
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Measuring differential expression using RNA-seq in organisms without a genome or transcriptome?"
I have NGS of single end read for non-model organism (control and experiemnt) done by commereical CLC bio. could anyone help me my quires. How differential expression analysis will be made. Sincee the contig of control library not match with the same contig of experimeent. any procedure or related publication for the same.
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modified 8.2 years ago
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Sequer • 150
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8.3 years ago by
Senthilkumar • 90
Senthil -- how's this rephrasing: I have RNA-seq data from a control and experimental condition for an organism that lacks a genome and I want to measure differential expression. I have already tried doing de novo assembly of the transcriptome separately on each sample but the contigs don't match, so I don't know how to compare them. Does anyone have any suggestions for how to assess differential expression with or without assembly of the transcriptome?
Also Senthil, try changing the title of your question. Maybe: "Measuring differential expression using RNA-seq in organisms without a genome or transcriptome?"
Can you rephrase this in an understandable question?