Methylation Analysis of NGS data generated by Roche GS Junior System
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8.2 years ago

Hi, has anybody experience in methylation analysis of data generated by Roche GS Junior system? I did bisulfit convertion and pyrosequencing with the GS Junior. I use the AVA-Software of Roche so far, which is suboptimal for this kind of questions. My problem is that the GS Junior outputs a sff-file. Is there any software for methylation analysis which deals with this sff-file? Or is there a way to transform my data, so that other software can read it?

sequencing alignment • 1.6k views
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