tophat-fusion-post multiple transcript fusions in a single hit?
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5.7 years ago
benformatics ★ 2.6k

Hello everyone,

I just finished pushing some fruit fly data through TopHat-Fusion and the post filtering part. I was noticing some peculiar things in the final results.html output files that were produced. For the most part everything looked good but in some cases individual result tables showed multiple genes:

3. chrX-chrX ff                  
nsonlytheo CG2930 chrX 3767208 ec chrX 3714758 3 0 0
nsonlytheo CG2930 chrX 3767208 ec chrX 3714758 5 0 0
nsonlytheo CG2930 chrX 3767208 ec chrX 3745969 11 0 0
nsonlytheo CG2930 chrX 3767208 ec chrX 3745969 4 0 0
nsonlytheo CG2930 chrX 3767208 ec chrX 3745969 6 0 0
nsonlytheo CG2930 chrX 3767208 ec chrX 3745969 9 0 0
mutantonlytheo CG2930 chrX 3767208 ec chrX 3745969 4 0 0
mutantonlytheo CG2930 chrX 3767208 ec chrX 3745969 10 0 0
mutantonlytheo CG2930 chrX 3767208 ec chrX 3745969 9 0 0
mutantonlytheo CG2930 chrX 3767208 ec chrX 3745969 4 0 0
mutantonlytheo CG2930 chrX 3767208 ec chrX 3745969 7 0 0
mutantonlytheo CG2930 chrX 3767208 ec chrX 3745969 8 0 0
mutantonlytheo CG2930 chrX 3767208 ec chrX 3745969 12 0 0
nsonlytheo tyf chrX 4014456 CG43689 chrX 3978842 3 0 0
mutantonlytheo tyf chrX 4014456 CG43689 chrX 3979650 3 0 0
mutantonlytheo tyf chrX 4014456 CG43689 chrX 3979650 4 0 0
mutantonlytheo tyf chrX 4014456 CG43689 chrX 3978842 3 0 0

 

                 

Any reason why I might expect to see something like this? The only obvious things are that both fusions are "ff" and between the same two chromosomes ?

Anybody else run into this?

RNA-Seq chimera tophat fusion tophat-fusion • 996 views
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