I have four conditions: wt, mut, wt-treat, mut-treat
where each of the 4 conditions have 8 time points (in duplicate), so 32 conditions, 64 samples
I would like to use DESeq2 with all the samples (create a dds object with all 64 samples), but be able to test for wt-0timept, mut-0timept, wt-treat-4hr, mut-treat-4hr in a block design. So, there are two different time points being tested.
I think I would know how to do this for a single time point, but I can not figure out how to do it when two time points (and 4 conditions, wt, mut, treat, no-treat) are in the comparison. Is this possible ?