Question: Help: Phred quality for ab1 files
gravatar for rabidbirdman4
3.7 years ago by
rabidbirdman40 wrote:


I'm using the biojava legacy release of 1.8.1 (API here:

I want to get the Phred quality values for single bases in an ab1 chromatogram. Using methods in the ABITools class, I was able to create a SymbolList of the sequence.


I'm now using the PhredTools class, but I don't know where to start.



ADD COMMENTlink modified 2.6 years ago by trausch1.4k • written 3.7 years ago by rabidbirdman40
gravatar for trausch
2.6 years ago by
trausch1.4k wrote:

Indigo analyses ab1 files and aligns them to a reference. The command-line version available here also dumps the chromatogram including the base-calls and the Phred quality values so this might be what you are looking for.

ADD COMMENTlink written 2.6 years ago by trausch1.4k
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