Question: Help: Phred quality for ab1 files
0
gravatar for rabidbirdman4
3.1 years ago by
rabidbirdman40 wrote:

Hello,

I'm using the biojava legacy release of 1.8.1 (API here: http://www.biojava.org/docs/api1.8/)

I want to get the Phred quality values for single bases in an ab1 chromatogram. Using methods in the ABITools class, I was able to create a SymbolList of the sequence.

 

I'm now using the PhredTools class, but I don't know where to start.

 

Thanks

ADD COMMENTlink modified 24 months ago by trausch1.2k • written 3.1 years ago by rabidbirdman40
0
gravatar for trausch
24 months ago by
trausch1.2k
Germany
trausch1.2k wrote:

Indigo analyses ab1 files and aligns them to a reference. The command-line version available here also dumps the chromatogram including the base-calls and the Phred quality values so this might be what you are looking for.

ADD COMMENTlink written 24 months ago by trausch1.2k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1150 users visited in the last hour