Entering edit mode
8.2 years ago
sibrunne
•
0
We're trying to enrich a Bacterial Artificial Chromosome (BAC) for sequencing by NGS. We'd like to enrich the vector from a gDNA prep or lysate prior to NGS to improve efficiency and coverage. Our idea is to use biotinylated oligos to hybridise with a unique region in the BAC and wash off any background genomic DNA.
Would anyone happen to have recommendations as for which protocol to use to design probes and to perform the enrichment reaction?
