I have manually created a customized database on NCBI+ tools on a command line centOS server. I have a blastx running against said database currently. My query being my transcriptome assembly.
My database consists of NR, uniprotKB and swissprot. Once my BlastX finishes and I obtain the gene information, E-values etc (I've selected
-outfmt 6); how do I go about obtaining GO classifications? My overall aim is to condense these GO terms with Plant GO SLIM afterwards, but I'm stuck on how to go from a blastx tabular formatted table to obtaining GO terms? Any help?
- I am not wanting to use Blast2GO due to time constraints and the fact you need to pay.
Additionally I wanted to do an IPS or PFAM classification, should I do this before the GO classification and combine both blastX results and protein results?