I am using Agilent sureselect to design probes for a particular gene. I got the missed regions in a bed file after finalizing design.I input these missed regions into the designs because I don't want to miss, as it is a promoter region of that gene. I came to see that Sureselect covers that region with a coverage of 75% which it said to have missed in the design with the entire coordinate of the gene. Can I go with that design(with missed region added)? Is 75% is a good coverage?
All answers are appreciated.