Question: Agilent Sureselect : Missed Regions picking up if we give gene coordinates seperately
gravatar for Amk
4.6 years ago by
Amk120 wrote:

Dear All,

I am using Agilent sureselect to design probes for a particular gene. I got the missed regions in a bed file after finalizing design.I input these missed regions into the designs because I don't want to miss, as it is a promoter region of that gene. I came to see that Sureselect covers that region with a coverage of 75% which it said to have missed in the design with the entire coordinate of the gene. Can I go with that design(with missed region added)? Is 75% is a good coverage?

All answers are appreciated.
Thank you.


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