I have a piece of 4Mbp of a chromosome of a plant species. I would like to see which genes are there in this piece of chromosome and what function do they have. I did a blast of a) Ensembl genes (CDS) of 10 orthologous species against my chromosome and found annotation using BioMart table, b) blast of UniprotKB SwissProt proteins of Viridiplantae against my chromosome and used the annotation table there. Do you have any suggestions if the methods I used are good? Or do you know any other ways how to annotate my 4Mbp long sequence?Any suggestions are welcomed.