Hello, I'm trying to detect SNPs and Indels in my RNAseq reads. I have mapped the reads to reference (BAM) and I have used Freebayes to call variants (VCF). I have used IGV viewer to the visualization and here is the problem. I load the genome and bam file without problems but I can't index the .vdf file using IVG. I obtain this error when I run the index option: Your input file has a malformed header: we never saw the required CHROM header line (starting with one#) for de input VCF file. My Freebayes version is 4.1.
Someone can help me??