Question: How are amino acid sequences obtained from proteomics studies?
1
gravatar for amango
4.1 years ago by
amango10
amango10 wrote:

Naive question here from a genomics person. How are proteomic results (e.g. from mass spec of extracted tissues) linked to annotated protein sequences? Can proteomic studies like mass spec directly give you the amino acid sequence, which you can then reverse-translate?

For example, if someone identifies the proteins in the glands of an insect, how do they go about getting sequences for those proteins?

ADD COMMENTlink modified 4.1 years ago • written 4.1 years ago by amango10
1
gravatar for genomax
4.1 years ago by
genomax80k
United States
genomax80k wrote:

Take a look at the first couple of videos for intro. to proteomic data analysis.
Ultimately you are doing similarity searches to identify the possible AA sequence. An example of the program used is Mascot.

ADD COMMENTlink modified 4.1 years ago • written 4.1 years ago by genomax80k

Exactly what I need, thanks.

ADD REPLYlink written 4.1 years ago by amango10
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