Question: Looking for pH-sensitive proteins
0
gravatar for kazenokizi
4.1 years ago by
kazenokizi30
United Kingdom
kazenokizi30 wrote:

Hello all,

I am looking for pH-sensitive proteins as models to test a hypothesis for my computational design. Could you suggest some proteins, preferably with PDB structures?

Thanks a lot.

Andrew

sensitivity protein ph • 991 views
ADD COMMENTlink modified 4.1 years ago by Michael Dondrup47k • written 4.1 years ago by kazenokizi30

Are you looking for proteins that "sense/respond" to pH (example)? Most proteins would be "sensitive" to big changes in pH.

ADD REPLYlink written 4.1 years ago by genomax80k

Yeah I agree with genomax2, most proteins are affected by changes in pH. Just like temperature (and water solubility) as well.

ADD REPLYlink written 4.1 years ago by Benn7.9k

Yes, I am looking for proteins which respond to pH changes by large conformational movement, but not into denaturation zone. I wonder whether there are some model proteins in which these responses are verified by experimental data, such as NMR, FRET, etc?

ADD REPLYlink written 4.1 years ago by kazenokizi30
2
gravatar for Michael Dondrup
4.1 years ago by
Bergen, Norway
Michael Dondrup47k wrote:

Vaults are large ribonucleoprotein particles found in most eukaryote cells consisting of the Major Vault Protein, two minor vault proteins and vault associated RNA. Upon exposure to low ph, it was shown that vaults separate into two halfs, the structure is available and the possible mechanism of vault opening and structure was described here.

ADD COMMENTlink modified 4.1 years ago • written 4.1 years ago by Michael Dondrup47k
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