I am new to Bioinformatics.I am trying to use GATK tool for finding SNP and indels but the problem is that the documentation seems to be complex for a beginner like me with so many tools to start with including IndelRealigner,MuTect,Haplotypecaller..The more I read the more confused I am getting.I also tried using Indel religner which is the first step in the process of identification of indels(correct me if wrong) but I got an error saying that you don't have the intervals file in the targetIntervals in the following command.
java -jar GenomeAnalysisTK.jar \ -T IndelRealigner \ -R reference.fasta \ -I input.bam \ -known indels.vcf \ -targetIntervals intervalListFromRTC.intervals \ -o realignedBam.bam
The error message is:Could not read file /home/aditya/Bioinfotools/gatk/intervalListFromRTC.intervals because The interval file does not exist. I don't know where to get the intervals file from.
But please guide me overall in understanding GATk.