hello everyone i did one experiment on microrna in epc cell which are derived from fathead minnow fish. i got the microrna sequences and now i need to do target prediction of microrna. the problem is there is a genome for fathead minnow but without annotation file, i checked the annotation file joined with the genome but doesn't contain any information about CDS or UTR positions. my idea is to use the transcriptome from this paper Transcriptome analysis of epithelioma papulosum cyprini cells after SVCV infection and do as they did in the paper. but i don't know how to get the 3'utr from transcriptome .can someone help me for this task because i need it in my project and i am a phd student in molecular biology so my bioinformatics knowledge is limited. thanks for your help.
Question: extract 3'UTR sequeunce from RNAseq data and genome reference
4.2 years ago by
najibveto • 40
Korea, Republic Of
najibveto • 40 wrote:
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