Question: Transcriptome assembly with variations among replicates
0
gravatar for Cacau
3.2 years ago by
Cacau430
Canada
Cacau430 wrote:

I have three biological replicates and want to assembly the transcriptome. How should I deal with the differences among replicates? For example, how to deal with different transcription start sites?

rna-seq assembly • 747 views
ADD COMMENTlink modified 3.2 years ago • written 3.2 years ago by Cacau430

It is the same sample and you want to get a comprehensive transcriptome representation, correct? Go with all three pooled together for assembly. You can individually align the samples to the transcriptome afterwards to judge differences (if any).

ADD REPLYlink modified 3.2 years ago • written 3.2 years ago by genomax68k

Yes, that is what I meant. I would also like to see whether they are consistent. Thanks a lot.

ADD REPLYlink written 3.2 years ago by Cacau430
0
gravatar for Cacau
3.2 years ago by
Cacau430
Canada
Cacau430 wrote:

Any help? Or should I only use one replicate?

ADD COMMENTlink written 3.2 years ago by Cacau430
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