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8.5 years ago
Cacau
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520
I have three biological replicates and want to assembly the transcriptome. How should I deal with the differences among replicates? For example, how to deal with different transcription start sites?
It is the same sample and you want to get a comprehensive transcriptome representation, correct? Go with all three pooled together for assembly. You can individually align the samples to the transcriptome afterwards to judge differences (if any).
Yes, that is what I meant. I would also like to see whether they are consistent. Thanks a lot.