Transcriptome assembly with variations among replicates
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7.5 years ago
Cacau ▴ 500

I have three biological replicates and want to assembly the transcriptome. How should I deal with the differences among replicates´╝č For example, how to deal with different transcription start sites?

RNA-Seq rna-seq Assembly • 1.4k views
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It is the same sample and you want to get a comprehensive transcriptome representation, correct? Go with all three pooled together for assembly. You can individually align the samples to the transcriptome afterwards to judge differences (if any).

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Yes, that is what I meant. I would also like to see whether they are consistent. Thanks a lot.

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7.5 years ago
Cacau ▴ 500

Any help? Or should I only use one replicate?

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