I'm new in analyzing RNA-Seq data coming from Illumina plateform. As a first step, I tried to fastqc only one file, and I obtained this result. As you can see, I got failure in 6 modules. I think that the Sequence Duplication Levels module (despite the failure) is ok in RNA-Seq data. My question is to know if I have to trim the first 13 bp from the reads (Per base sequence content show the failure) or not ? And I need some advices from you about the kmer content failure.