Question: Statistcal Effectnof Using Pooled Samples For Rna Seq
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gravatar for Mkd
7.4 years ago by
Mkd0
Mkd0 wrote:

We have RNA seq data with three biological replicates for both control and treatment. Due to the nature of the experiment, each biological replicate is actually a pool of material from 30 different individuals. Does this alter which algorithms are best to detect differential gene expression between the samples since I would expect that our design greatly reduced stochastic variation (there is very little variation in expression Among biological replicates for genes expressed highernthan 1 RPMK)

differential rna statistics • 2.9k views
ADD COMMENTlink written 7.4 years ago by Mkd0
1

@mkd: Out of curiosity, does this mean that your three replicates span a pool of 90 different people? Or are you constructing three different libraries from the same pool of 30 people?

ADD REPLYlink written 7.4 years ago by Steve Lianoglou5.0k
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