What Ngs Read Simulators Are Available For Single-End 35Base Pair Length Data?
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12.4 years ago
Chintzz • 0

I want to generate small RNA data for illumina.

Can anyone suggest the tool to simulate 35 basepair length data for illumina in fastq or fasta format?

I have tried ART (set of simulation tools). But the mapping data is not as expected for small RNA data.

small mirna simulation next-gen sequencing • 3.5k views
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Can you explain "mapping data is not as expected for small RNA data"?

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More specifics on how you generated the data are warranted, probably.

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You can use the NGS:Simulation tool, available here Galaxy

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@pascal: only 1 or 2 reads mapped on the complete genome.I would say there wasnt any mapping...!! @Sean Davin: According to me atleast 3 reads should map onto mature miRNA to prove that it is present!!

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12.4 years ago
Zhidkov ▴ 600

Take a look at previous thread,[?] NGS:Simulation tool, available here Galaxy[?]
There are several other tools availble for fastq simulation:[?] Mason[?] simulateSeq.pl[?]

Ilia

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