I am complete newbie in bioinformatics and not friendly with command line applications. Recently I stumbled in a problem with BLAST search of protein query in genomes via tblastn. The online BLAST-server does not have all needed genomes, so I decided to run search in my computer. I downloaded genomes of different organisms in archives, unzipped them and renamed for convenience. All files are initially in *.fna or *.fasta extensions were saved as *.fna for convenience as in NCBI databases and were sorted with taxonomic position as criteria into different folders. Which free available program with GUI can be used for tblastn search of protein homologs inside downloaded genomes, and what special tips to pay attention. One of the problems I encounter when trying to use UGENE is that it ask to make database and then starts to pour errors.
Question: BLAST search with protein query in assembly file with .fna extension.
4.8 years ago by
biophoenix • 0
biophoenix • 0 wrote:
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