Question: conversion of nucleotide sequence to aminoacid sequence
0
gravatar for ashamscsoft
3.3 years ago by
ashamscsoft20
ashamscsoft20 wrote:

I have one nucleotide sequence. I need to change that sequence to amino acid sequence. To convert there are many tools, but how do we know that the sequence converted to amino acid sequence is correct or not. I'm getting different sequence when i try it in different tools. Could someone help in this.

translation • 2.3k views
ADD COMMENTlink modified 3.3 years ago by archana.bioinfo87160 • written 3.3 years ago by ashamscsoft20

What organism is the gene sequence from? Are there introns? What tools have you tried? What do the outputs look like?

ADD REPLYlink written 3.3 years ago by jotan1.2k

The organism is homo sapiens. The sequence is cDNA sequence in that there wont be introns. I tried in expasy, EMBL-EBI and sequence manipulation suite and other normal conversions.

ADD REPLYlink written 3.3 years ago by ashamscsoft20

Seems pretty straightforward, it's hard to understand what might be going wrong. Could you show the sequence and/or conversions?

ADD REPLYlink written 3.3 years ago by jotan1.2k

Hi ashamscsoft, if you need more specific help please add an example to your question of a sequence that is translated differently. There are few parameters that influence translation by tools like transeq, see also A: Translating DNA to protein with perl for reasons where implementation could differ.

However, if the genetic code is the same, the frame and strand are the same, and the input is the same, then the output of all tools should also be identical. If not maybe one or all are at fault.

ADD REPLYlink written 3.3 years ago by Michael Dondrup46k

thanks will check it

ADD REPLYlink written 3.3 years ago by ashamscsoft20
0
gravatar for archana.bioinfo87
3.3 years ago by
archana.bioinfo87160 wrote:

You can use emboss tool for DNA to protein conversion using http://web.expasy.org/translate/ Hope it will help you.

ADD COMMENTlink written 3.3 years ago by archana.bioinfo87160

I have a doubt in that tool if we give our sequences, it gives the output as frames. how we wil get full amino acid sequences L C V I T A Q Q K K F H S D A Q K F L K F W R I S D R L C H Y V F T Y C Q like this

ADD REPLYlink written 3.3 years ago by ashamscsoft20
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