I am trying to use BUSCO to assess the quality of a transcriptome assembled with Trinity. My run fails after ~2h running, when the HMMER phase begins. I couldn't find any support options for BUSCO.
Is there any way to get support for BUSCO? I couldn't find anything on line or on the documentation.
This is my command and programs versions:
module load Python/3.2.1 module load BLAST+/2.2.31 module load HMMER/3.1b2 module load EMBOSS/6.5.7 python3 ~/MyPrograms/BUSCO_v1.1b1/BUSCO_v1.1b1.py -in ../Trinity-GG.fasta -o GG \ -l ~/MyPrograms/BUSCO_v1.1b1/vertebrata -m trans -c 4 -f
After ~2 hours running, this is the error I get:
*** Running HMMER to confirm transcript orthology *** Traceback (most recent call last): File "/.../MyPrograms/BUSCO_v1.1b1/BUSCO_v1.1b1.py", line 594, in <module> name=i[:-7];group=transdic[name] KeyError: 'TRINITY_'
Any help would be appreciated