Entering edit mode
7.9 years ago
grayapply2009
▴
280
Hi guys, I've finished annotating my de novo assembled transcriptome with blast2go and exported the results to a fasta file. The format of the fasta file is like this:
>Seq1|e3 ubiquitin-protein ligase rnf213 isoform x2|GO:1234 GO:4567 GO:7890
SEQUENCE
>Seq2|transcription factor hivep2|GO:4321 GO:7654 GO:8970
SEQUENCE
The gene names/description and GO terms (separated by "|") are assigned by blast2go. My situation is that I am not allowed to use the gene names assigned by blast2go. How do you guys get gene names for you sequences? What methods/tools do you use to assign accurate gene names to you sequences?