I have a query sequence with 1935 bp and a reference sequence with 2817 bp. My query aligns fully with the reference sequence. I need to extract the regions from the reference sequence(2817bp) which are not a part of my query i.e.approximately 882 bp. I need to do this for many such files (nearly 200). Please help me with a script if possible. I couldn't find any tools online for the same. Kindly help!
Question: How to extract a region from a reference sequence which does not align with a given query read?
19 months ago by
pradyumna Sagar • 20
Manipal, School Of Life Sciences
pradyumna Sagar • 20 wrote:
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