Hi Fellow Bioinformatician,
I am doing de novo RNASeq assembly using Velvet/Oases for a few publicly available RNASeq datasets. They are paired end 101bp reads generated by Illumina. I read and found that Oases has a parameter for insert length (-ins_length), but I have no idea how much the insert length is and I have following questions:
Can I run Oases on paired end RNASeq data without inputing an insert length ? And what would be the default behavior in that case ?
Is there any way to find out insert length for publicly available paired end RNASeq data if it's not provided in the description or article ?
Thanks for your help!