Question: Cross Reactor Script
0
gravatar for User 7351
8.6 years ago by
User 735140
User 735140 wrote:

Hey guys I need a Python script or other script I can reverse engineer to search BLAST to check for primer cross reactivity between species (forward primer, reverse primer, 5k max bp between primer/probe hybridization). Most of these scripts are proprietary. Will anyone help me out with this or brainstorm with me how to design such a script?

primer • 1.2k views
ADD COMMENTlink written 8.6 years ago by User 735140
1
gravatar for Sean Davis
8.6 years ago by
Sean Davis26k
National Institutes of Health, Bethesda, MD
Sean Davis26k wrote:

Not a direct answer to your question, but I wanted to make sure you were aware of this tool:

http://genome.ucsc.edu/cgi-bin/hgPcr

Access to the source code is available here:

http://genome.ucsc.edu/admin/git.html

ADD COMMENTlink modified 13 months ago by RamRS30k • written 8.6 years ago by Sean Davis26k

Cool. I am building a new all-in-one tool. I may have to interface with that.

ADD REPLYlink written 8.6 years ago by User 735140
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