I have read many questions related to this answer but still I doubt about it. my data looks like below
Treatment Controlled Gene 1 0.23 0.48 Gene 2 0.65 0.76 Gene 3 0.88 0.66 Gene 4 0.48 0.065 ....
I don't have any replicate and I want to find which gene is up regulated and which one is down regulated. I do know that I cannot get much info when i don't have any replicate but I want to know if there is any way to get up/down regulated genes based on statistical test ?
for example what if I get the fold change (treatment/controlled) and then I put a cutoff and select the genes based on that ?