Question: finding up or down regulated gene expression
gravatar for Learner
4.7 years ago by
Learner 230
Learner 230 wrote:

I have read many questions related to this answer but still I doubt about it. my data looks like below

              Treatment     Controlled 
Gene 1    0.23              0.48
Gene 2    0.65              0.76
Gene 3    0.88               0.66
Gene 4     0.48             0.065

I don't have any replicate and I want to find which gene is up regulated and which one is down regulated. I do know that I cannot get much info when i don't have any replicate but I want to know if there is any way to get up/down regulated genes based on statistical test ?

for example what if I get the fold change (treatment/controlled) and then I put a cutoff and select the genes based on that ?

limma gene expression R • 1.4k views
ADD COMMENTlink modified 4.7 years ago by WouterDeCoster45k • written 4.7 years ago by Learner 230
gravatar for WouterDeCoster
4.7 years ago by
WouterDeCoster45k wrote:

Without replicates there is no valid statistical test which can reliably give you results about differential gene expression.

ADD COMMENTlink written 4.7 years ago by WouterDeCoster45k

@decosterwouter this is obvious ! please read above, these stuff are said million times. I am looking for another solution instead hearing what is already said over 10 times

ADD REPLYlink written 4.7 years ago by Learner 230
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